Free shipping on orders over $150Multi-vial pricing on research volume ordersThird-party HPLC tested · CoAs rolling out across the catalogueSupplied for laboratory and research purposes onlyFree shipping on orders over $150Multi-vial pricing on research volume ordersThird-party HPLC tested · CoAs rolling out across the catalogueSupplied for laboratory and research purposes onlyFree shipping on orders over $150Multi-vial pricing on research volume ordersThird-party HPLC tested · CoAs rolling out across the catalogueSupplied for laboratory and research purposes only
Home/Lab tests/Reconstitution calculator
Laboratory handling

Peptide reconstitution & concentration calculator

Work out the concentration of a reconstituted vial, plan aliquots to avoid repeated freeze–thaw, and convert between mass and volume for a working dilution. Everything updates as you type.

This is concentration arithmetic for laboratory handling. It does not recommend quantities and it is not guidance for human use — Mr. Peps supplies for laboratory and research purposes only.

Vial and solvent
Optional — or just type the mass below.
mL
Bacteriostatic water for multi-use, sterile water for single use.
Optional
g/mol
From the CoA or the supplier's spec sheet. Gives you molarity.
mL
Splitting the vial to avoid repeated freeze–thaw.
Standard C₁V₁ = C₂V₂ arithmetic — the volume of this solution that holds the mass you enter.
Concentration
Concentration5 mg/mL
Also expressed as5000 µg/mL
Per microlitre5 µg/µL
Molarity—
10 mg ÷ 2 mL = 5 mg/mL
Aliquots
Full aliquots—
Peptide per aliquot—
Left over—
Transfer volume
Volume required—
Before you reconstitute

The five things that ruin a vial

Full handling guide →
Cold vial, warm room

Let the vial reach room temperature before opening. Solvent hitting cold lyophilised powder is the most common cause of a cloudy reconstitution.

Down the wall, not onto the cake

Angle the vial and run the solvent down the glass. A jet fired straight into the powder shears the peptide.

Swirl, never shake

Roll it gently between your fingers. Shaking denatures peptides and foams the solution; foam is lost material.

Give it time

Some peptides take several minutes to go fully into solution. Leave it before deciding something is wrong.

Aliquot once

Every freeze–thaw cycle costs you peptide. Split the vial while it's fresh and thaw each aliquot once.

For laboratory and research purposes only.

Mr. Peps supplies research-grade peptides for in vitro and laboratory research. Not for human consumption. Not therapeutic. Not for diagnostic use. Compliance with applicable local research and import regulations is the customer's responsibility.